Protective effect of Na2SeO3 against cerebral ischemia-reperfusion injury to the hippocampal neurons in rats / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the protective effects of selenium on rat hippocampal neurons against ischemia-reperfusion (IR) injury.</p><p><b>METHODS</b>Thirty-two rats were randomly divided into sham-operated group, IR group and selenium-treated group, and in the latter two groups, cerebral IR injury was induced by middle cerebral artery occlusion; Na2SeO3 treatment was administer in selenium-treated group. At 14 days after reperfusion, the brain tissues were harvested from the rats and hippocampal neuron injuries were observed by TUNEL and Methylene Blue staining. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and nerve growth factor (NGF) in the hippocampal tissues were measured by ELISA.</p><p><b>RESULTS</b>Compared with IR group, the rats in selenium-treated group showed no significant increase in the expression of m-NGF (P>0.05), but pro-NGF expression was significantly increased (P<0.05) in the hippocampal tissue. Na2SeO3 treatment significantly inhibited the expressions of TNF-α and IL-1β and decreased the apoptosis of hippocampal neurons following cerebral IR injury (P<0.05).</p><p><b>CONCLUSION</b>Selenium produces antiapoptotic effect to protect the hippocampal neurons following cerebral IR injury possibly not by increasing the level of m-NGF but by decreasing the expressions of the inflammatory factors.</p>

Analysis of personality traits on felons / 法医学杂志

OBJECTIVE@#To study the personality traits of felons and their relevant factors, and to recommend for criminal psychological correction and crime prevention.@*METHODS@#Using Minnesota Multiphasic Personality Inventory (MMPI) to test felons in initial and middle stage of sentence serving.@*RESULTS@#The differences of MMPI factors between felon group and normal group were statistically significant (P < 0.001). The felon group's raw scores in F, Pd, Pa, Pt and Sc factors were significantly higher than those of the normal group. The felons whose T score of Pd was higher than that of the normal group by one standard deviation had the largest proportion. In subscales of F, Hs, D, Hy, Pd, Pa, Pt, Sc and Ma, the proportion of people whose T score higher than the normal was relatively large. The supernormal proportion in the two-point code type was large.@*CONCLUSION@#Felon group is a special group with high-risk personality disorder. It is important to take more correctional research on them.

Protective effect of penehyclidine hydrochloride in cerebral ischemia-reperfusion injury rats / 中华急诊医学杂志

Objective To investigate the effect of a selective muscarinic receptor antagonist (penehyclidine hydrochloride) in three vessel occlusion model of acute global cerebral ischemia-reperfusion in rats.Method One hundred and forty-four male SD rats were randomly divided into four groups:sham operated group,vehicle treated group (saline 1 ml,i.p.),scopolamine treated group (0.01 mg/kg,i.p.) and penehyclidine hydrochloride treated group (0.01 mg/kg,i.p.) with drugs injected 40 minutes before ischemia respectively.The ischemic duration was 10 minutes.The animals were subjected to motor activity tests (open field activity test,beam-walking test and grip test) at 24 hours or on the 3rd and 7th day after reperfusion.HE staining,TUNEL staining and immunohistochemical reactions of bax and bel-2 were carried out at the time points of 2,12,24 hours,3 and 7 days after reperfusion.TTC staining was carried out in some rats for assessment of infarction volume on the 4th day after reperfusion.Results As compared with the vehicle treated group,both penehyclidine hydrochloride treatment and scopolamine treatment decreased the numbers of apeptotie neurons (P